## Laboratory & Data Analysis # End-to-end service for monitoring antibiotic resistance ## laboratory for amr ### Complete quantitative AMR analysis service Resistomap offers specialised laboratory and data analysis services for monitoring and researching environmental antimicrobial resistance (AMR). Samples are sent directly to our laboratory in Helsinki for analysis. We provide clear, comparative data by quantifying AMR genes in eDNA using the HT-qPCR method. Our services are available to anyone interested in understanding AMR, including universities, research institutions, public health organisations, environmental agencies, and more. 600+ Primer sets for targeted gene quantification designed, with the option of additional customisation. 10 days Analysis results available within approximately ten business days from sample's arrival at our laboratory. Want to discuss how our service could benefit your research? ## Services for research ### DNA extraction ✓ Sampling protocols and kits. ✓ DNA extraction from any environmental sample type. ✓ Shipping solutions. ### HT-qPCR analysis ✓ Customisable gene targets (600+). ✓ Analysis configurations range between 384 Genes in 4 Samples to 6 Genes in 256 Samples. Results in 10 working days from sample arrival at our lab. ### Data visualisation ✓ Compare across samples. ✓ Downloadable graphics for reporting and showcasing. ✓ Interactive visualisations. The Resistomap Platform research view Explore real data from a study tracking carbapenem resistance and hospital-acquired infection genes, based on samples from two hospitals in Finland’s capital region. ## How it works **Sampling services:** **Sampling Kit & Shipping** Our specialisation lies in analysing environmental samples and quantifying the presence of AMR in any type of environment. **Optional services include:** - **Detailed sampling protocols and tutorial videos.** - **For water sampling, we can provide kits equipped with 0.22 µm pore-sized PES filter units for effective water sample filtration.** - **Global pick-up and temperature-controlled transportation options to securely deliver your samples to our laboratory facilities.** **Extraction services:** **Quality controlled standards** Customers can send their samples to us in extracted eDNA form or as unprocessed environmental samples. For an additional fee, we offer a DNA extraction service using advanced, standardised procedures to ensure high-quality DNA from environmental samples. **Analysis:** **High-Throughput qPCR** The workhorse of our service is HT-qPCR technology, a method designed to quantify genes with both precision and efficiency. **Results:** **Visualised and interactive** Results are delivered promptly within 10 working days and can be provided as raw data or accessed through the Research View of the Resistomap Platform. ## Selection of publications Our service has been utilised by researchers all over the globe for various studies and fields of research. This is a small selection and you can find our complete list linked below. **Water research** - Tavares, R. D. S., Fidalgo, C., Rodrigues, E. T., Tacão, M.,& Henriques, I. (2024). Integron-associated genes are reliable indicators of antibiotic resistance in wastewater despite treatment- and seasonality-driven fluctuations. _Water Research, 258_, 121784. [https://doi.org/10.1016/j.watres.2024.121784](https://doi.org/10.1016/j.watres.2024.121784) - Robins, K., O'Donnell, G., Neumann, A., Schmidt, W., Hart, A., & Graham, D. W. (2024). Antimicrobial resistance in rural rivers: Comparative study of the Coquet (Northumberland) and Eden (Cumbria) River catchments. _Science of The Total Environment, 928_, 172348. [https://doi.org/10.1016/j.scitotenv.2024.172348](https://doi.org/10.1016/j.scitotenv.2024.172348) **Public health** - Samarra, A., Cabrera-Rubio, R., Martínez-Costa, C., & Collado, M. C. (2024). Unravelling the evolutionary dynamics of antibiotic resistance genes in the infant gut microbiota during the first four months of life. _Annals of Clinical Microbiology and Antimicrobials, 23_, Article 72. [https://doi.org/10.1186/s12941-024-00725-z](https://doi.org/10.1186/s12941-024-00725-z) - Magalhães, E. A., de Jesus, H. E., Pereira, P. H. F., Gomes, A. S., & dos Santos, H. F. (2024). Beach sand plastispheres are hotspots for antibiotic resistance genes and potentially pathogenic bacteria even in beaches with good water quality. _Environmental Pollution, 344_, 123237. [https://doi.org/10.1016/j.envpol.2023.123237](https://doi.org/10.1016/j.envpol.2023.123237) **Agriculture** - Zalewska, M., Błażejewska, A., Szadziul, M. _et al._ Effect of composting and storage on the microbiome and resistome of cattle manure from a commercial dairy farm in Poland. _Environ Sci Pollut Res_ **31**, 30819–30835 (2024). [https://doi.org/10.1007/s11356-024-33276-z](https://doi.org/10.1007/s11356-024-33276-z) - Jauregi, L., González, A., Garbisu, C., & Epelde, L. (2023). Organic amendment treatments for antimicrobial resistance and mobile element genes risk reduction in soil-crop systems. _Scientific Reports, 13_, Article 863. [https://doi.org/10.1038/s41598-023-27871-3](https://doi.org/10.1038/s41598-023-27871-3) **FAQs** Find answers to the most common questions about our monitoring service process, gene selection, DNA concentrations and other important information. Which genes do you measure? Can we choose the target genes? - We have over 600 previously validated primer sets available in our database. A single SmartChip analysis can measure up to 384 genes. Do you detect pathogens? Yes, at the moment we have 26 primer sets available to target certain taxonomy groups of bacteria and pathogens. Which sample types do you accept? We accept all types of samples, e.g., soil, sediment, sludge, manure, stool, meat, tissue, swabs, river, lake, wastewater, and seawater. How long is the analysis process? We deliver analysis results within 10 working days. For three or more chips, the analysis process may take longer. What services do you offer? In Resistomap we provide antibiotic resistance monitoring services starting from sampling to the final analysis of the SmartChip qPCR results.